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Sections were collected in 0.1 M phosphate-buffered saline (PBS) and blocked for 1.5 h at room temperature in a blocking solution containing 0.3% Triton X-100, 2% donkey serum, and the appropriate concentration of goat serum in 0.1 M PBS

Diabetes 59 , 10301037 (2010)

L2-Cmu development and validation To target IGF-1R action in mice, we utilized the mAb, L2-Cmu (Amgen Inc, Thousand Oaks, CA), which is a murinized IgG1 version of the fully human L2-C mAb previously reported by Calzone et al

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